<100>Describe the procedure or methods for how to "open up" a cell through a process called "electroporation." <101>Describe the procedure or methods for exact reactions that take place when you do glutathione S-transferase (GST) cleavage during affinity chromatography. <102>Describe the procedure or methods for different quantities of different components to use when pouring a gel to make it more or less porous. <103>Describe the procedure or methods for green fluorescent protein (GFP) tagged proteins to do experiments with tagged proteins. <104>Describe the procedure or methods for how to do a microsomal budding assay, i.e., budding of vesicles from microsomes in vitro. <105>Describe the procedure or methods for purification of rat IgM. <106>Describe the procedure or methods for chromatin IP (Immuno Precipitations) to isolate proteins that are bound to DNA in order to precipitate the proteins out of the DNA. <107>Describe the procedure or methods for normalization procedures that are used for microarray data. <108>Describe the procedure or methods for identifying in vivo protein-protein interactions in time and space in the living cell. <109>Describe the procedure or methods for fluorogenic 5'-nuclease assay. <110>Provide information about the role of the gene Interferon-beta in the disease Multiple Sclerosis. <111>Provide information about the role of the gene PRNP in the disease Mad Cow Disease. <112>Provide information about the role of the gene IDE gene in the disease Alzheimer's Disease. <113>Provide information about the role of the gene MMS2 in the disease Cancer. <114>Provide information about the role of the gene APC (adenomatous polyposis coli) in the disease Colon Cancer. <115>Provide information about the role of the gene Nurr-77 in the disease Parkinson's Disease. <116>Provide information about the role of the gene Insulin receptor gene in the disease Cancer. <117>Provide information about the role of the gene Apolipoprotein E (ApoE) in the disease Alzheimer's Disease. <118>Provide information about the role of the gene Transforming growth factor-beta1 (TGF-beta1) in the disease Cerebral Amyloid Angiopathy (CAA). <119>Provide information about the role of the gene GSTM1 in the disease Breast Cancer. <120>Provide information on the role of the gene nucleoside diphosphate kinase (NM23) in the process of tumor progression. <121>Provide information on the role of the gene BARD1 in the process of BRCA1 regulation. <122>Provide information on the role of the gene APC (adenomatous polyposis coli) in the process of actin assembly. <123>Provide information on the role of the gene COP2 in the process of transport of CFTR out of the endoplasmic reticulum. <124>Provide information on the role of the gene casein kinase II in the process of ribosome assembly. <125>Provide information on the role of the gene Nurr-77 in the process of preventing auto-immunity by deleting reactive T-cells before they migrate to the spleen or the lymph nodes. <126>Provide information on the role of the gene P53 in the process of apoptosis. <127>Provide information on the role of the gene alpha7 nicotinic receptor subunit gene in the process of ethanol metabolism. <128>Provide information on the role of the gene gamma-aminobutyric acid receptors (GABABRs) in the process of inhibitory synaptic transmission. <129>Provide information on the role of the gene Interferon-beta in the process of viral entry into host cell. <130>Provide information about the genes BRCA1 regulation of ubiquitin in cancer. <131>Provide information about the genes L1 and L2 in the HPV11 virus in the role of L2 in the viral capsid. <132>Provide information about the genes APC (adenomatous polyposis coli) and wnt in colon cancer. <133>Provide information about the genes phospholipase A2 (PLA2) and SAR1 in Endoplasmic reticulum transport (i.e. vesicle budding from the ER). <134>Provide information about the genes CFTR and Sec61 in degradation of CFTRwhich leads to cystic fibrosis. <135>Provide information about the genes Bop and Pes in cell growth. <136>Provide information about the genes alpha7 nicotinic receptor gene and ApoE gene in the neurotoxic effects of ethanol. <137>Provide information about the genes Insulin-like GF and insulin receptor gene in the function in skin. <138>Provide information about the genes HNF4 and COUP-TF I in the suppression in the function of the liver. <139>Provide information about the genes Ret and GDNF in kidney development. <140>Provide information about BRCA1 185delAG mutation and its/their role in ovarian cancer. <141>Provide information about Huntingtin mutations and its/their role in Huntington's Disease. <142>Provide information about Sonic hedgehog mutations and its/their role in developmental disorders. <143>Provide information about Mutations of NM23 and its/their impact on tracheal development. <144>Provide information about Mutations in metazoan Pes and its/their effect on cell growth. <145>Provide information about Mutations of hypocretin receptor 2 and its/their role in narcolepsy. <146>Provide information about Mutations of presenilin-1 gene and its/their biological impact in Alzheimer's disease. <147>Provide information about Mutations of alpha7 nAChR gene and its/their biological impact in alcoholism. <148>Provide information about Mutation of familial hemiplegic migraine type 1 (FHM1) and its/their neuronal Ca2+ influx in hippocampal neurons. <149>Provide information about Mutations of the alpha 4-GABAA receptor and its/their impact on behavior.